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. 2021 Oct 26;9:753196. doi: 10.3389/fcell.2021.753196

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Copyright © 2021 Li, Chen, Li, Wu, Ye, Tian, Wei, Hao, Pan, Zhou, Yang, Fu, Xu and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PGLS regulated the migration and invasion of HCC. (A) Representative images of CFUs in migration assay. (B) CFU clone number per field on Huh7 in migration assay. Invaded cells from five representative fields were counted. (C,D) The number of invasive cells significantly decreased after the treatment of these three siRNAs, respectively (n = 5). (E) Wound healing in Huh7 cells treated with siRNA-1,siRNA-2, and siRNA-3. The lines indicated the edge of wound at 0 and 24 h. (F) Migration rate was analyzed and expressed as the area of cells migrating from the original wounds. Data show individual values and mean ± SD. (B,D,F) Unpaired two-tailed Student t-tests, assessed statistical significance, ***p < 0.001.