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. 2021 Jun 14;70(9):2107–2119. doi: 10.2337/db20-0591

Figure 8.

Figure 8

TLR2 KO reverses high-fat diet–induced impairment in endothelium-dependent vasorelaxation. Eight-week-old male WT and Tlr2−/− mice were randomly divided into two dietary groups and fed either regular chow or high-fat diet for 6 weeks. Freshly isolated aortic rings were mounted into an organ bath (Multi Wire Myograph System) under 12 mN tension for 5 min and then equilibrated by PSS bicarbonate buffer. The rings were precontracted with 1 μmol/L phenylephrine and then exposed to incremental concentrations of acetylcholine (1 nmol/L–1 μmol/L) or sodium nitroprusside (1 nmol/L–1 μmol/L) to evaluate endothelium-dependent or endothelium-independent vasorelaxation, respectively. A: Grouped data of endothelium-dependent vasorelaxation in response to acetylcholine in precontracted aortic rings (n = 10–11). Data are shown as mean ± SEM. **P < 0.01, ***P < 0.001 vs. WT + regular chow; ###P < 0.001 vs. WT + high-fat diet. B: Grouped data of endothelium-independent vasorelaxation in response to sodium nitroprusside in precontracted aortic rings (n = 5–7). Data are shown as mean ± SEM. Differences are nonsignificant among groups.