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. 2021 Jul 7;70(9):2014–2025. doi: 10.2337/db20-0888

Figure 2.

Figure 2

Vpr induces conversion of subcutaneous WAT to BAT-like phenotype. Ucp1 mRNA is increased in inguinal WAT of Vpr-Tg (n = 6–8) compared with WT (n = 5–8) mice (A) and in sVpr-treated (n = 7–8) compared with vehicle-treated (n = 7–8) mice (B). Ucp1 expression is similar in BAT of Vpr-Tg (n = 6–8) compared with WT (n = 5–8) mice (C) and in sVpr-treated (n = 7–8) compared with vehicle-treated (n = 7–8) mice (D). Protein expression of UCP1 is increased in iWAT of Vpr-Tg compared with WT mice (E) and in sVpr-treated compared with vehicle-treated mice (F). G: Hematoxylin-eosin (H&E) stain shows transformation to a BAT phenotype in iWAT of Vpr-Tg compared with WT mice and in sVpr-treated compared with vehicle-treated mice. H: UCP1 expression (immunohistochemistry) is increased in iWAT of Vpr-Tg compared with WT mice and sVpr-treated compared with vehicle-treated mice, and in BAT of sVpr-treated compared with vehicle-treated mice. Images were cropped at similar magnification. PC, positive control BAT. Two-tailed, unpaired t tests for unequal variance were used. P < 0.05 was considered significant. Values are mean ± SD. **P < 0.01.