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. 2021 Oct 26;11:740557. doi: 10.3389/fonc.2021.740557

Figure 3.

Figure 3

Icaritin moderated the proliferation and Warburg effect of HCC cells via regulating the expression of FAM99A. (A) The heatmap shows the transcriptome sequencing of differentially expressed lncRNAs in HCC cells treated with icaritin (10 μm). (B) The expression of FAM99A was detected by qRT-PCR after HepG2 and HCCLM3 cells were transfected with pcDNA3.1/FAM99A. (C) The GLUT1 mRNA expression and protein levels in HCC cells were detected after the overexpression of FAM99A by qRT-PCR and Western blotting assays. (D, E) Glucose consumption and lactate production of HepG2 and HCCLM3 cells were detected. (F) MTT assay showed that the overexpression of FAM99A could lead to decreased cell viability of HCC cells. (G) The expression of FAM99A was detected. (H–J) Cell viability, GLUT1 expression, glucose consumption and lactate production of HCC cells transfected with si-FAM99A were detected. *P < 0.05, **P < 0.01.