Figure 4.

FAM99A moderated the Warburg effect through the JAK2/STAT3 pathway. (A–C) The mRNA expression of JAK2 and STAT3, gp130, and gp80, and protein level of JAK2/p-JAK2, STAT3/p-STAT3, gp130, and gp80 in HCC cells were detected after the overexpression of FAM99A. (D) The mRNA expression and protein level of STAT3 in HCC cells were detected by qRT-PCR and Western blotting assays. (E) GLUT1 expression was detected by qRT-PCR and Western blotting assays after the HCC cells were transfected with vector pcDNA3.1/fam99a or pcDNA3.1/FAM99A+pcDNA3.1/STAT3. ^** p < 0.01, ns, no significance.