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. 2021 Oct 26;11:740557. doi: 10.3389/fonc.2021.740557

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Copyright © 2021 Zheng, Gou, Jiang, Yang, Yang and Qin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FAM99A blocked the translation of gp130 and gp80 via binding to EIF4B. (A) Western blotting assay was used to detect the protein levels of gp130 and gp80 in HCC cells after the transfection of pcDNA3.1/FAM99A and treatment of MG132. (B) Silver staining and differential band mass spectrometry assays were conducted to analyze the binding protein pulled down by FAM99A. (C) Western blotting verified the presence of EIF4B and JAK2 in the products pulled down by biotinylated FAM99A. (D) RIP and qRT-PCR assays were conducted to detect the enrichment of FAM99A, gp130, and gp80 in EIF4B precipitates in HepG2 cells. *P < 0.05, ^** p < 0.01.