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FAM99A inhibited STAT3 phosphorylation to affect the JAK2/STAT3 activity via binding to JAK2. (A) RIP and qRT-PCR assays were conducted to verify whether FAM99A could bind to JAK2. (B) We adopted RIP and qRT-PCR assays to verify whether FAM99A could bind to FLAG with the JH1 catalytic domain. (C) Western blotting assay was conducted to detect the effect of FAM99A on phosphorylated STAT3. ** p < 0.01.
