. 2021 Oct 26;12:767140. doi: 10.3389/fpls.2021.767140

TABLE 1.

Stable transformation efficiency of transgenic plants from cotyledon explants of B. rapa.

Total no. of infected cotyledon explants	Total no. of shoots survived under third selection	Total no. of rooted shoots	Total no. of GUS positive plants	Total no. of Southern blot positive plants	Stable transformation efficiency (%)
128	64.0^b	38.0^b	24.0^b	18.0^a	14.0^a
128	72.0^a	42.0^a	26.0^a	19.0^a	14.8^a
128	58.0^c	34.0^c	21.0^c	17.0^a	13.2^a

Values represent the mean of three experiments. Transformation efficiency = (number of southern positive plants/total number of infected cotyledon explants)/100. ^a−cMeans with the same letter are not significantly different at 5% level according to Duncan’s multiple range test.