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. 2021 Oct 26;8:728273. doi: 10.3389/fmolb.2021.728273

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Copyright © 2021 Lima, Kolliari-Turner, Malinsky, Guppy, Martin, Wang, Voss, Georgakopoulos, Borrione, Pigozzi and Pitsiladis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Yield (A) , relative yield (B) , RIN (C), and absorbance ratio (D) of RNA extracted from whole blood samples using the five different protocols. RIN, RNA integrity number; Temp, Tempus protocol; EDTA + Temp, K₂EDTA tube plus Tempus protocol; GJ_aliq, GeneJET aliquoted protocol; EDTA + GJ, K₂EDTA tube plus GeneJET protocol; GJ_fresh, Fresh blood and GeneJET protocol. The relative yield was reached by multiplying the final value of GeneJET protocol RNA extraction by 12 (as the starting volume is 12-fold lower than that of the Tempus protocol); * = p ≤ 0.05; ** = p ≤ 0.001. The graphs show mean values ±SD.