FIG 3.

Impact of E2 mutations on the fitness of Asian lineage CHIKV in Aedes aegypti. Aedes aegypti mosquitoes were fed artificial blood meals with approximately equal genomic ratios of wt and E2 mutant CHIKV Mal06. At 10 dpi, heads were collected, and their virus mixture was passaged for 2 days in Vero cells. The resulting cell culture supernatant was amplified by RT-PCR, and the ratio of wt to mutant CHIKV was determined by Sanger sequencing. Relative replicative fitness values were calculated as described in Materials and Methods. The Holm-Sidak correction for multiple P values was applied. ns, not significant (P ≥ 0.05); ***, P < 0.001; ****, P < 0.0001.