TABLE 2.

List of the top 50 human proteins putatively enriched at the Toxoplasma parasitophorous vacuole membrane^a

Accession no.	Description	Gene	mT-RAH + Toxo/mT-RAH + mock log2 FC
Q5VSL9	Striatin-interacting protein 1	STRIP1	2.13
O75340-2	Isoform 2 of programmed cell death protein 6	PDCD6	2.00
A0A0C4DH65	Protein KRBA1	KRBA1	1.72
Q8NHP6	Motile sperm domain-containing protein 2	MOSPD2	1.70
Q6P1N0	Coiled-coil and C2 domain-containing protein 1A	CC2D1A	1.67
Q6N043	Zinc finger protein 280D	ZNF280D	1.66
P49750	Isoform 4 of YLP motif-containing protein 1	YLPM1	1.53
Q5T0F9	Coiled-coil and C2 domain-containing protein 1B	CC2D1B	1.51
A6NMQ1	DNA polymerase	POLA1	1.46
Q6P1N0-2	Isoform 2 of coiled-coil and C2 domain-containing protein 1A	CC2D1A	1.41
Q6PJI9	GATOR complex protein WDR59	WDR59	1.39
Q99816	Tumor susceptibility gene 101 protein	TSG101	1.37
Q7Z460	CLIP-associating protein 1	CLASP1	1.18
Q99570	Phosphoinositide 3-kinase regulatory subunit 4	PIK3R4	1.18
Q99661	Kinesin-like protein KIF2C	KIF2C	1.16
Q9NYA1-2	Isoform 2 of sphingosine kinase 1	SPHK1	1.13
P21580	Tumor necrosis factor alpha-induced protein 3	TNFAIP3	1.12
Q07960	Rho GTPase-activating protein 1	ARHGAP1	1.05
Q8NDZ4	Deleted in autism protein 1	DIPK2A	0.97
Q9P273	Teneurin-3	TENM3	0.96
Q9Y2K6	Ubiquitin carboxyl-terminal hydrolase 20	USP20	0.95
Q8TEY7	Ubiquitin carboxyl-terminal hydrolase 33	USP33	0.85
P15408	Fos-related antigen 2	FOSL2	0.84
P17275	Transcription factor jun-B	JUNB	0.78
P15407	Fos-related antigen 1	FOSL1	0.75
Q8WUM4	Programmed cell death 6-interacting protein	PDCD6IP	0.72
Q14BN4	Sarcolemmal membrane-associated protein	SLMAP	0.71
Q9UK41	Vacuolar protein sorting-associated protein 28 homolog	VPS28	0.67
Q86VY9	Transmembrane protein 200A	TMEM200A	0.67
P18850	Cyclic AMP-dependent transcription factor ATF-6 alpha	ATF6	0.65
Q6UWP8	Suprabasin	SBSN	0.64
O94822-3	Isoform 3 of E3 ubiquitin-protein ligase listerin	LTN1	0.64
O75154-3	Isoform 3 of Rab11 family-interacting protein 3	RAB11FIP3	0.62
P53985	Monocarboxylate transporter 1	SLC16A1	0.62
Q96F4	Interleukin-17 receptor A	IL17RA	0.62
Q9NU22	Midasin	MDN1	0.59
Q96JA1	Leucine-rich repeats and immunoglobulin-like domains protein 1	LRIG1	0.57
P35475	Alpha-l-iduronidase	IDUA	0.50
O95983	Methyl-CpG-binding domain protein 3	MBD3	0.49
Q5D862	Filaggrin-2	FLG2	0.48
Q9NYI0	PH and SEC7 domain-containing protein 3	PSD3	0.48
P32189	Glycerol kinase	GK	0.46
Q9BTX7	Alpha-tocopherol transfer protein-like	TTPAL	0.45
P53794	Sodium/myo-inositol cotransporter	SLC5A3	0.44
P30048	Thioredoxin-dependent peroxide reductase mitochondrial	PRDX3	0.43
Q9C0C4	Semaphorin-4C	SEMA4C	0.43
P40189	Interleukin-6 receptor subunit beta	IL6ST	0.43
P29590-2	Isoform PML-5 of Protein PML	PML	0.43
P02786	Transferrin receptor protein 1	TFRC	0.42
Q9H444	Charged multivesicular body protein 4b	CHMP4B	0.42

^aThe relative abundance of each protein was determined across all samples using the peptide-level TMT quantification, and the log₂ fold change (log₂ FC) was determined for various sample ratios. The proteins were first filtered by requiring a minimum log₂ FC of −0.65 for the (mT-RAH + Toxo)/(mT + Toxo) ratio and a log₂ FC of 0 for the (mT-RAH + Toxo/mT-RAH + Toxo, no biotin) ratio (see Materials and Methods). Displayed here are the top 50 human proteins (identified by at least 2 unique peptides, with a protein score of >25, and with a protein coverage of >1%) ranked according to the log₂ FC between the averaged mT-RAH + Toxo samples versus mT-RAH + mock samples. Also shown are their majority human UniProt identifiers (“Accession Number” column), i.e., the proteins that contain at least half of the peptides belonging to a group of proteins that cannot be unambiguously identified by unique peptides, the descriptive name for each protein (“Description” column), and the gene symbol (“Gene” column). The proteins of unknown location in infected cells chosen for follow-up localization studies are bolded.