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. 2021 Nov 9;12(6):e02684-21. doi: 10.1128/mBio.02684-21

FIG 10.

FIG 10

macroH2A1 and SMRT corepressor independently localize to replication foci. (A) HPV31 (9E cells), HPV16 wild-type cells, and HPV16 ΔE8^E2 genome-containing cells were immunostained with antibodies against RPA (purple), macroH2A1.2 (green), and SMRT (red). A white dotted line outlines the nucleus. A high-resolution image of a deconvolved image is shown from a single slice of Z stacks collected throughout the nucleus for the 9E cell. The magnified image in the box area demonstrates macroH2A1.2 and SMRT localization at replication foci. (B) The percentage of foci enriched for SMRT and macroH2A1.2 in differentiated cells containing HPV31 (n = 50 9E cells, RPA-positive foci = 159), HPV16 wild-type (n = 28 cells, RPA-positive foci = 29), and HPV16ΔE8^E2 (n = 53 cells, RPA-positive foci = 241) was analyzed. Data are from two independent biological replicates. (C) Fluorescence intensity scans obtained by drawing a line through the nuclei indicated using Leica LAS X software. A gray bar above the scan delineates the position of the replication focus.