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. 2021 Oct;9(20):1573. doi: 10.21037/atm-21-4978

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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PLOD1-targetting miR-449 is low expressed in AMSCs. (A) PLOD1 protein levels were examined in mouse and human AMSCs by ELISA. MEFs and HSFs treated with/without TGF-β1 were used as controls. (B) Bioinformatics tools were used to find 3 PLOD1-targeting miRNAs conserved in mouse and human. (C) RT-qPCR for the 3 PLOD1-targeting miRNAs in mouse and human AMSCs. (D,E) The binding sites of miR-449 on human (D) and mouse (E) PLOD1 3'-UTR are shown. *, P<0.05. N=5. ns, non-significant; MEFs, mouse embryonic fibroblasts; HSFs, human skin fibroblasts; AMSCs, adipose-derived mesenchymal stem cells; miRNA, microRNA; RT-qPCR, quantitative reverse transcription polymerase chain reaction; TGF-β1, transforming growth factor-β1; 3'-UTR, 3 prime untranslated region.