FIG. 2.

Several poly(A)⁺ RNA transport mutants show a defect in heat shock protein synthesis at 42°C. SDS-PAGE of total cellular proteins labeled with [³⁵S]-methionine in wild-type (WT) and various mutant strains treated for 30 min at 25 or 42°C is shown. The positions of the stress-inducible yeast heat shock proteins Hsp104p, Hsp82p, and Hsp70s are shown by arrows. (B) Wild-type heat shock protein labeling pattern is restored upon transformation of rna1-1 with a plasmid carrying the wild-type RNA1 gene.