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. 2021 Jan 5;69(5):1241–1250. doi: 10.1002/glia.23960

FIGURE 1.

FIGURE 1

In vitro viability of adenoviral vector (AVV)‐transduced cultured astrocytes expressing lactate oxidase (LOx) or a control reporter. Trypan Blue exclusion assay (a) and XTT assay (b) consistently reported no effect of AVV‐sGFAP‐LOx‐IRES‐tdTomato on cell viability below an AVV MOI of 50; cell viability was unaffected by transduction with reporter (EGFP) expressing control AVV across the range of tested MOI ratios. (a) Sample sizes n = 8 wells in No AVV, n = 5 and n = 6 wells in Control MOI 15 and 50, respectively, and n = 6 and n = 5 wells in LOx MOI 15 and MOI 50, respectively. (b) Sample sizes n = 6 wells in no AVV, and n = 9 wells in Control and LOx at all MOIs tested. ANOVA (Bonferroni's Multiple Comparison Test) for comparison between control AVV transduction and un‐transduced astrocytes; unpaired two‐tailed t‐test for comparison of LOx to control reporter expressing astrocytes at each MOI tested [Color figure can be viewed at wileyonlinelibrary.com]