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. 2021 Nov 5;221(1):e202010178. doi: 10.1083/jcb.202010178

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© 2021 De-Castro et al.

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Figure 5. — The integrity and gating function of the TZ are maintained in wdr-60 mutants but compromised in the xbx-1 mutant. (A) Analysis of the localization of several components of the MKS (TMEM-107::GFP and MKS-6::mCherry) and NPHP (GFP::NPHP-4) modules of the TZ in phasmid cilia of the indicated strains. (B) Quantification of MKS-6::mCherry signal intensity confined at the TZ and dispersed along cilia (n ≥ 38 cilia). (C) Relative localization of the nonciliary membrane protein RPI-2::GFP to the TZ (labeled with MKSR-1::tdTomato) in phasmid cilia of the indicated strains. (D) Signal overlap between these components and quantification of the amount of RPI-2::GFP leaking into cilia (n ≥ 33 cilia). Gray rectangles highlight the TZ region, defined by MKS-6 and MKSR-1 localization. XY intensity distribution graphs are shown as mean ± SEM. Scale bars: 2 µm.