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. 2021 Nov 5;221(1):e202010178. doi: 10.1083/jcb.202010178

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© 2021 De-Castro et al.

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Figure S1. — Schematic of the C27F2.1 locus organization in the C. elegans genome. Related to Figs. 1 and 2. Predicted wdr-60 exons (in gray boxes), including the start and stop codons, and the X-box motif according to Blacque et al. (2005). (A) Knock-in insertion of the ::3xflag::gfp sequence at the 3′ end of the wdr-60 genomic sequence (in-frame with the WDR-60 coding sequence). (B) Representation of the wdr-60(tm6453) null allele. (C) Representation of the wdr-60(ΔCT) allele (also named dan1(Δ1498-4125 bps)) corresponding to a WDR-60 truncation of the C-terminal β-propeller domain (Δ288–668 aa).