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. 2021 Nov 9;19:168. doi: 10.1186/s12958-021-00851-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — ICA administration improved impaired seminiferous tubule in diabetic rats. A Hematoxylin and eosin staining of testicular tissue. The lower panes show partial amplification of upper panes. In each seminiferous tubule, the black arrow points to a representative spermatogonia, the red arrow, primary spermatocyte, and the green arrowhead, Sertoli cell. In addition, the green curves present regions used to analyze the cross-sectional area of seminiferous tubule whose value displayed beside. B The area of seminiferous tubules from different treatment groups. C Quantification of spermatogonia, primary spermatocytes and Sertoli cells from different treatment groups. N = 6. Bars assigned with different superscripts mean significant difference (p < 0.05)