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. 2021 Nov 9;19:168. doi: 10.1186/s12958-021-00851-9

Fig. 5.

Fig. 5

ICA administration upregulated the expression of SIRT1 and HIF-1α in the testis of diabetic rats. A Immunohistochemical analysis of SIRT1 expression, and red arrows show representatively positive signals of spermatogonia. B mRNA expression analysis of Sirt1 with qRT-PCR. D Immunohistochemical analysis of HIF-1α expression. Red arrows show representatively positive signals of spermatogonia. D mRNA expression analysis of Hif-1α with qRT-PCR. E Western blotting analysis of SIRT1 and HIF-1α protein expression. Bars assigned with different superscripts mean significant difference (p < 0.05)