Table 1.
Primer pairs and corresponding annealing temperatures used in this study
| Gene | Sequence(5′-3′) | Annealing Temperature | Product (bp) |
|---|---|---|---|
| PCNA |
F: GCTCCATCCTGAAGAAGGT R: TGCACTAAGGAGACGTGAGA |
55 °C | 121 |
| Bax | F:GAGACACCTGAGCTGACCTT | 55 °C | 104 |
| R:TCCATGTTGTTGTCCAGTTC | |||
| Bcl-2 | F:AGTACCTGAACCGGCATCT | 55 °C | 120 |
| R:TCTTCAGAGACAGCCAGGA | |||
| Caspase-3 | F:CCGGTTACTATTCCTGGAGA | 55 °C | 117 |
| R:TAACACGAGTGAGGATGTGC | |||
| SIRT1 |
F:GTGGCAGTAACAGTGACAGTG R:GTCAGCTCCAGATCCTCCAG |
55 °C | 143 |
| HIF1α |
F:GATGGAATGGAGCAGAAGAC R: CACAATCGTAACTGGTCAGC |
55 °C | 112 |
| CAT |
F: ACAACTCCCAGAAGCCTAAGAATG R: RGCTTTTCCCTTGGCAGCTATG |
55 °C | 76 |
| Gpx |
F: GGAGAATGGCAAGAATGAAGA R: RCCGCAGGAAGGTAAAGAG |
55 °C | 139 |
| SOD |
F: CGAGCATGGGTTCCATGTC R: CTGGACCGCCATGTTTCTTAG |
55 °C | 101 |
| GAPDH |
F: CCTCAAGATTGTCAGCAATG R: CAGTCTTCTGAGTGGCAGTG |
55 °C | 134 |