Fig. 6.

miR-378a-3p is hypermethylated by DNMT1 and silenced in HCC tissues and cells. A, B miR-378a-3p mRNA expression was detected in HCCLM3 and SMMC-7721 cells treated with 10 μM 5-Aza-dC for 48 h. C, D HCCLM3 and SMMC-7721 cells were transfected with DNMTs siRNA for 48 h, DNMTs mRNA expression was measured by qRT-PCR. E, F HCCLM3 and SMMC-7721 cells were transfected with DNMTs siRNA for 48 h, and miR-378a-3p mRNA expression was measured. G, H miR-378a-3p mRNA expression was detected in HCC cells transfected with pcDNA-DNMT1 and pcDNA-NC. I, J The mRNA expression of DNMT1 in the TCGA database and in 10 paired HCC and adjacent normal tissues is shown. K The relationship between DNMT1 and miR-378a-3p in 10 matched fresh HCC samples is shown. *P < 0.05; **P < 0.01; ***P < 0.001