Table 1.

Sex differences in PAG ECS proteome

	Accession	Max fold change over other sex	Anova (p)
Detected greater in female	ABHD6_MOUSE*	1.51	0.03
	MGLL_MOUSE*	1.44	0.01
	NAPEP_MOUSE	1.34	0.93
	PLD3A_MOUSE	1.11	0.46
	PLD2_MOUSE	1.18	0.56
	DGLB_MOUSE	1.05	0.56
	DGLA_RAT;DGLA_MOUSE	1.01	0.85
Detected greater in male	PHLD_MOUSE	1.52	0.15
	PLD1_RAT;PLD1_MOUSE	1.18	0.30
	PLD3_MOUSE	1.07	0.40
	PLD6_MOUSE	1.15	0.84
	CNR1_RAT	2.49	0.13
	CNRP1_MOUSE;CNRP1_RAT	1.02	0.82
	ABD12_MOUSE	1.02	0.79

Summary of endocannabinoid system (ECS) enzymes detected in greater quantity in either female or male PAG samples, as measured by quantitative proteomics. The 2-AG hydrolyzing enzymes MAGL and ABHD6 (bold) were detected at significantly higher fold-detection for females vs males (*ABHD6 female vs. male p = 0.03, MAGL female vs. male p = 0.01). Italics indicate study via IHC. CNR2 and FAAH were below the level of detection in this study.

Protein gene abbreviations: ABHD6 alpha/beta-hydrolase domain containing 6; MGLL monoacylglycerol lipase; NAPEP N-acyl-phosphatidylethanolamine-specific phospholipase D; PLD3A phospholipase D3A; PLD2 phospholipase D2; DGLB diacylglycerol lipase beta; DGLA diacylglycerol lipase alpha; PHLD phosphatidylinositol-glycan-specific phospholipase D; PLD1 phospholipase D1; PLD3 phospholipase D3; PLD6 phospholipase D6; CNR1 cannabinoid receptor 1; CNRP1 cannabinoid receptor interacting protein 1; ABD12 alpha/beta-hydrolase domain containing 12