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. Author manuscript; available in PMC: 2021 Nov 9.
Published in final edited form as: Sci Immunol. 2021 Mar 5;6(57):eabe3218. doi: 10.1126/sciimmunol.abe3218

Fig. 6. Enhanced ILC2 responses and accelerated expulsion of N. brasiliensis after ACh treatment.

Fig. 6.

(A to G) Numbers of total ILC2s (A), representative flow cytometry plots (B and E), population frequencies (C and F) and numbers (D and G) of IL-5+ (B to D) and IL-13+ (E to G) ILC2s in the lungs of PBS or ACh-treated mice analyzed on Day 7 of N. brasiliensis infection. (H to L) Representative flow cytometry plots (H), population frequencies (I and K) and numbers (J and L) of eosinophils (I and J) and Siglec Fhigh activated eosinophils (K and L) in the lungs of PBS or ACh-treated mice analyzed on Day 7 of N. brasiliensis infection. Data are pooled from three independent experiments. n = 13 mice per group. (M and N) Representative sections (M) and goblet cell numbers (N) of small intestine from PBS or ACh-treated mice analyzed on Day 7 of N. brasiliensis infection with periodic acid–Schiff (PAS)–Alcian blue staining. Data are pooled from two independent experiments. n = 6 mice per group. (O) Worm counts in the small intestine of PBS or ACh-treated mice analyzed on day 7 of N. brasiliensis infection. Data are pooled from two independent experiments. n = 9 mice per group. Data are mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001.