Table 3.
Primers used for one-step quantitative real-time PCR.
| Genes | Gene symbols | Forward primers (5′-3′) | Reverse primers (5′-3′) | Probes | Accession number |
|---|---|---|---|---|---|
| Fatty acid synthase | Fas | GCCAGCGTGCCATGTTC | GGGCCGTTGAAGTCAAAGAA | CCAACCGACTCTCC | XM-021576228 |
| Fatty acid binding protein | Fabp | AGAGAAGTTCTCCCACATCAGAGA | CTTGCTTATCCTGGTGAGGGTT | TCACTGCGGCCTCAAC | XM-020500137.2 |
| Fatty acid elongase | Fae | CTGATGTACTCTTACTATGGGCTCTCT | AACTGAATCAGCTGTACTTGTGTGA | CCTTGCGGCCCTATCT | AY605100 |
| Delta-5- desaturase | CTGGGCCACATCC | CCAAAGCCAGGGCCTCTAG | GCCTCCTGTTCTTCAGCCTCTA | XM-014170388 | |
| Delta-6- desaturase | GACCATGTTTTCACAACGGAACTG | CAGAAGAAGCGAAGGTAG AAAGTCA | ACCACGCCAGATCCA | AY458652 | |
| β-actin | bact | CCCTCTTCCAGCCCTCCT T | AGTTGTAGGTGGTCT CGTGGATA | CCGCAA GACTCC ATACCGA | AF254414 |