. 2021 Oct 11;7(4):1360–1370. doi: 10.1016/j.aninu.2021.07.008

Table 6.

Fatty acid composition of the total lipid fraction (% of total fatty acids) in the muscle of rainbow trout fed experimental diets for 10 weeks¹.

Item	Name	FO	SO	BSLO	BSLO + BA	P-value
Saturates
C14:0	Myristic acid	3.35 ± 0.07^b	0.82 ± 0.04^c	4.86 ± 0.18^a	4.71 ± 0.31^a	<0.001
C16:0	Palmitic acid	14.64 ± 0.81^c	17.84 ± 0.65^b	22.87 ± 0.39^a	23.54 ± 0.51^a	<0.001
C18:0	Stearic acid	4.96 ± 0.09^b	6.43 ± 0.33^a	5.91 ± 0.10^a	6.21 ± 0.21^a	<0.001
ΣSFA		22.95 ± 0.80^c	25.09 ± 0.90^b	33.65 ± 0.53^a	35.13 ± 0.64^a	<0.001
Monoenes
C16:1n-7	Palmitoleic acid	5.23 ± 0.08^a	1.56 ± 0.05^c	3.18 ± 0.08^b	3.24 ± 0.39^b	<0.001
C18:1n-9	Oleic acid	23.38 ± 0.19^a	17.57 ± 0.52^b	18.64 ± 0.37^b	19.43 ± 1.37^b	<0.001
C20:1n-9	11-Eicosenoic acid or Gondoic acid	12.56 ± 0.62^a	1.34 ± 0.03^b	1.04 ± 0.04^b	1.00 ± 0.10^b	<0.001
C22:1n-9	Erucic acid	5.69 ± 0.78^a	ND	ND	ND	<0.001
ΣMUFA		46.86 ± 1.48^a	20.47 ± 0.52^b	22.86 ± 0.41^b	21.99 ± 0.88^b	<0.001
Polyunsaturate
C18:2n-6	linoleic acid	8.50 ± 0.19^c	30.13 ± 1.47^a	18.82 ± 0.44^b	17.41 ± 1.07^b	<0.001
C18:3n-3	α-Linolenic acid	1.13 ± 0.07^b	4.13 ± 0.17^a	1.28 ± 0.06^b	1.50 ± 0.24^b	<0.001
C20:2n-6	Eicosadienoic acid	0.48 ± 0.01^d	1.82 ± 0.08^a	1.32 ± 0.03^b	1.09 ± 0.07^c	<0.001
C20:3n-6	Dihomo-γ-linolenic acid	0.26 ± 0.00^c	1.62 ± 0.09^a	1.46 ± 0.05^ab	1.33 ± 0.07^b	<0.001
C20:4n-6	Arachidonic acid	0.75 ± 0.03^c	1.78 ± 0.15^b	2.31 ± 0.12^a	2.00 ± 0.23^ab	<0.001
C20:5n-3	Eicosapentaenoic acid	6.29 ± 0.09^a	1.87 ± 0.15^b	1.90 ± 0.11^b	2.13 ± 0.18^b	<0.001
C22:5n-3	Docosapentaenoic acid	1.98 ± 0.08^a	0.79 ± 0.05^c	1.02 ± 0.08^b	0.82 ± 0.07^bc	<0.001
C22:6n-3	Docosahexaenoic acid	11.42 ± 1.10^b	12.78 ± 1.20^ab	15.39 ± 0.67^a	15.43 ± 1.09^a	0.017
ΣPUFA		30.19 ± 0.79^c	54.44 ± 0.68^a	43.49 ± 0.67^b	42.88 ± 1.18^b	<0.001
EPA + DHA		17.70 ± 1.15	14.65 ± 1.33	17.29 ± 0.75	17.56 ± 1.24	0.233
EPA:DHA		0.58 ± 0.04^a	0.15 ± 0.01^b	0.12 ± 0.00^b	0.14 ± 0.01^b	<0.001
DHA:EPA		1.81 ± 0.16^c	6.82 ± 0.29^b	8.20 ± 0.33^a	7.37 ± 0.39^ab	<0.001
Σn-3 PUFA		20.81 ± 1.05	19.57 ± 1.20	19.59 ± 0.76	19.17 ± 1.39	0.735
Σn-6 PUFA		9.38 ± 0.38^c	34.87 ± 1.55^a	23.91 ± 0.29^b	21.71 ± 1.33^b	<0.001
n-3:n-6 ratio		2.28 ± 0.21^a	0.58 ± 0.06^b	0.82 ± 0.04^b	0.90 ± 0.07^b	<0.001

FO = fish oil; SO = soybean oil; BSLO = black soldier fly oil; BA = bile acid; SFA = saturated fatty acid; ND = not detected; MUFA = monounsaturated fatty acid; PUFA = polyunsaturated fatty acid; EPA = eicosapentaenoic acid; DHA = docosahexaenoic acid.

Data expressed as mean ± SE, n = 9; values along row with different superscripts differ significantly (P < 0.05). Initial average weight of fish is 32 ± 0.0 g.