. 2021 Oct 11;7(4):1360–1370. doi: 10.1016/j.aninu.2021.07.008

Table 7.

Fatty acid composition of the total lipid fraction (% of total fatty acids) in rainbow trout liver fed experimental diets for 10 weeks¹.

Item	Name	FO	SO	BSLO	BSLO + BA	P-value
Saturates
C14:0	Myristic acid	2.04 ± 0.07^b	0.55 ± 0.02^c	4.05 ± 0.26^a	3.60 ± 0.30^a	<0.001
C16:0	Palmitic acid	15.10 ± 0.26^b	13.79 ± 0.20^c	15.44 ± 0.23^ab	16.07 ± 0.45^a	<0.001
C18:0	Stearic acid	6.74 ± 0.14^c	8.52 ± 0.12^a	8.01 ± 0.25^ab	7.75 ± 0.17^b	<0.001
C20:0	Arachidic acid/eicosanoic acid	ND	0.15 ± 0.01^a	0.11 ± 0.01^b	0.12 ± 0.00^b	<0.001
ΣSFA		23.88 ± 0.26^b	23.00 ± 0.22^b	27.60 ± 0.41^a	27.55 ± 0.30^a	<0.001
Monoenes
C16:1n-7	Palmitoleic acid	2.64 ± 0.23^a	0.70 ± 0.03^b	2.83 ± 0.27^a	2.28 ± 0.32^a	<0.001
C18:1n-9	Oleic acid	17.42 ± 1.01^a	12.37 ± 0.33^b	20.54 ± 1.05^a	18.00 ± 1.76^a	<0.001
C20:1n-9	11-Eicosenoic acid or Gondoic acid	6.27 ± 0.35^a	1.79 ± 0.08^c	2.90 ± 0.17^b	2.49 ± 0.26^b	<0.001
C22:1n-9	Erucic acid	1.56 ± 0.13^a	0.10 ± 0.01^b	0.17 ± 0.02^b	0.14 ± 0.01^b	<0.001
ΣMUFA		27.90 ± 1.69^a	14.96 ± 0.41^c	26.44 ± 1.44^ab	22.91 ± 2.30^b	<0.001
Polyunsaturate
C18:2n-6	Linoleic acid	3.05 ± 0.11^c	15.84 ± 0.54^a	10.65 ± 0.44^b	10.54 ± 0.27^b	<0.001
C18:3n-3	α-Linolenic acid	0.34 ± 0.01^b	1.02 ± 0.06^a	0.25 ± 0.02^c	0.22 ± 0.01^c	<0.001
C18:3n-6	γ-Linolenic acid	ND	0.24 ± 0.01^a	0.16 ± 0.01^c	0.22 ± 0.07^b	<0.001
C20:2n-6	Eicosadienoic acid	0.84 ± 0.03^c	4.40 ± 0.23^a	2.52 ± 0.08^b	2.37 ± 0.14^b	<0.001
C20:3n-6	Dihomo-γ-linolenic acid	0.33 ± 0.01^d	2.99 ± 0.10^a	2.53 ± 0.08^b	2.31 ± 0.08^c	<0.001
C20:4n-6	Arachidonic acid	3.33 ± 0.19^b	7.48 ± 0.34^a	6.36 ± 0.36^a	6.94 ± 0.51^a	<0.001
C20:3n-3	Eicosatrienoic acid	0.16 ± 0.02^b	0.38 ± 0.01^a	0.10 ± 0.01^c	0.09 ± 0.01^c	<0.001
C20:5n-3	Eicosapentaenoic acid	7.59 ± 0.21^a	2.48 ± 0.10^b	1.35 ± 0.08^c	1.55 ± 0.11^c	<0.001
C22:5n-3	Docosapentaenoic acid	1.91 ± 0.04^a	0.65 ± 0.02^b	0.46 ± 0.03^c	0.50 ± 0.04^c	<0.001
C22:6n-3	Docosahexaenoic acid	30.67 ± 1.40^a	26.56 ± 0.85^b	21.57 ± 1.46^c	24.82 ± 1.75^bc	0.001
ΣPUFA		48.23 ± 1.57^b	62.04 ± 0.34^a	45.96 ± 1.49^b	49.55 ± 2.07^b	<0.001
EPA + DHA		38.25 ± 1.53^a	29.03 ± 0.88^b	22.92 ± 1.53^c	26.37 ± 1.85^bc	<0.001
EPA:DHA		0.25 ± 0.01^a	0.09 ± 0.00^b	0.06 ± 0.00^c	0.06 ± 0.00^c	<0.001
DHA:EPA		4.04 ± 0.15^c	10.84 ± 0.52^b	16.02 ± 0.53^a	16.07 ± 0.50^a	<0.001
Σn-3 PUFA		40.68 ± 1.50^a	31.08 ± 0.84^b	23.74 ± 1.56^c	27.16 ± 1.86^bc	<0.001
Σn-6 PUFA		7.55 ± 0.12^c	30.96 ± 0.54^a	22.22 ± 0.16^b	22.38 ± 0.34^b	<0.001
n-3:n-6 ratio		5.39 ± 0.17^a	1.01 ± 0.04^b	1.07 ± 0.07^b	1.21 ± 0.08^b	<0.001

FO = fish oil; SO = soybean oil; BSLO = black soldier fly oil; BA = bile acid; SFA = saturated fatty acid; MUFA = monounsaturated fatty acid; ND = not detected; PUFA = polyunsaturated fatty acid; EPA = eicosapentaenoic acid; DHA = docosahexaenoic acid.

Data expressed as mean ± SE, n = 9; values along row with different superscripts differ significantly (P < 0.05). Initial average weight of fish is 32 ± 0.0 g.