Figure 2.

Characterization of purified recombinant ADAMTS8.A, characterization of purified WT ADAMTS8 and ADAMTS8 EQ. The purified proteins were analyzed by reducing SDS-PAGE, followed by immunoblotting using either anti-FLAG or anti-ADAMTS8 antibodies. Protein purity was assessed by Coomassie Brilliant Blue (CBB). B, ADAMTS8 WT was subjected to SDS-PAGE under reducing (R) or non-reducing (N) conditions, followed by immunoblotting using anti-FLAG antibody. C, to detect N-linked glycosylation in ADAMTS8, WT and EQ ADAMTS8 were treated with (+) or without (−) PNGase before reducing SDS-PAGE (4–12%) and immunoblotting. Red asterisks indicate potential autolytic products; black asterisks indicate possible aggregates of ADAMTS8. Z and M indicate the zymogen and mature forms, respectively. Representative blots from at least n = 3 are presented.