Figure 3.

ADAMTS autocatalysis.A, schematic showing how ADAMTS8 WT and EQ semi-tryptic and/or tryptic peptides with differential abundance were used to identify putative autolytic cleavage sites. Digestion of a general substrate is shown. This can be either a different protein or, as presented in (B–D), ADAMTS8 itself to define autolytic products. B, all identified N-terminal or C-terminal (∗) semi-tryptic peptide abundance was compared to obtain a ratio (WT/EQ) and z-score. Cleavage sites are identified by amino acid and residue number with the hyphen indicating the scissile bond. C, all tryptic peptides were compared by quantified ratio (EQ/WT) and z-score. For both (A and B) a z-score >2 was considered significant (specifics can be found in Table S1). D, ADAMTS8 domain map showing the location of the putative cleavage sites (red arrows) and tryptic peptides (dashed black brackets).