Figure 4.

Endocytosis of ADAMTS8 by LRP1 WT and LRP1-knockout MEFs. WT and LRP1 knockout (KO) MEFs were cultured for 8 h in the presence (+) or absence (−) of either ADAMTS8 or ADAMTS5 (20 nM). Medium was harvested, trichloroacetic acid-precipitated, and resuspended in SDS sample buffer containing 5% mercaptoethanol. Following SDS-PAGE and immunoblotting, ADAMTS5 and 8 were detected by an anti-FLAG antibody. Band intensity was quantified by densitometry. Intensity of the bands corresponding to either ADAMTS8 or ADAMTS5 before incubation (pre) was set as 100%. A, immunoblot showing three biological replicates. B, densitometric analysis of FLAG-reactive bands. Data are presented as mean ± SD. In the case of ADAMTS5, only a single biological replicate is shown.