Figure 1.

ALIX domain organization and summary of recombinant ALIX constructs used in the current work.A, schematic of ALIX organization. Primary sequence of the PRD is shown. B, recombinant ALIX constructs used in the current work. Constructs 1 to 3 were obtained from Addgene; accession no. 80641 (47), 17639 (48), and 42577 (49), respectively. Construct 4 is described in our previous work (16); Addgene accession no. 141344. The positions of purification tags are marked. TEV protease cutting sites are shown. The point mutation, P801G, of $\mathrm{P}\mathrm{R}{\mathrm{D}}_{\mathrm{703}–\mathrm{868}}^{\mathrm{S}\mathrm{t}\mathrm{r}\mathrm{e}\mathrm{p}}$ is marked. C, SDS-PAGE analysis of TEV-cleaved ALIX constructs; the order of the constructs is the same as the one depicted in B. ALIX, apoptosis-linked gene-2 interacting protein X; PRD, proline-rich domain; TEV, tobacco etch virus.