Figure 4.

¹⁵N–{¹H} heteronuclear NOE data for nonphosphorylated and phosphorylated Bro1. Regions with high local mobility on the nanosecond time scale, ¹⁵N–{¹H} NOE <0.5, are labeled and marked with semitransparent gray rectangles. The location of Y319 is indicated by a vertical dashed line. All data were acquired with 500 μM ²H/¹⁵N labeled proteins at a spectrometer ¹H frequency of 800 MHz at 30 °C.