Figure 1.

Subcellular localizations of RB, IPI-O1, and IPI-O4.

Microscopy analysis shows that both RB and IPI-O1 are localized in the cytoplasm and the nucleus, whereas IPI-O4 is primarily localized in the cytoplasm. RB, IPI-O1, or IPI-O4 fused to GFP at the C terminus was expressed in N. benthamiana. Confocal images were taken at 48 h post infiltration (hpi) (left). DAPI staining depicts nuclei in blue (middle). An overlay of GFP and DAPI fluorescence signals is shown on the right. Scale bars correspond to 50 μm. The experiments were repeated three times with similar results.