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IPI-O1 and IPI-O4 differentially affect self-association of the RB CC domain.
Total proteins were extracted from N. benthamiana plants expressing RB CC-3×FLAG and RB CC-4×Myc together with 3×HA-IPI-O1 or 3×HA-IPI-O4. Immunoprecipitation was performed with an anti-Myc antibody, and immunoblots were probed with anti-FLAG, anti-Myc, or anti-HA antibodies. Ponceau S staining of immunoblots served as a loading control. The experiments were repeated three times with similar results.
