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IPI-O1 interacts with itself and IPI-O4 in planta.
Total proteins were extracted from N. benthamiana plants expressing 4×Myc-tagged IPI-O1 or IPI-O4 together with 3×FLAG-tagged IPI-O1 or IPI-O4. Immunoprecipitation was performed with an anti-Myc antibody, and immunoblots were probed with anti-FLAG or anti-Myc antibodies. Ponceau S staining of immunoblots served as a loading control. Asterisks indicate nonspecific bands. The experiments were repeated twice with similar results.
