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. 2021 Aug 25;2(6):100236. doi: 10.1016/j.xplc.2021.100236

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

IPI-O4 perturbs the self-association of IPI-O1 but enhances the association of RB CC with IPI-O1.

(A) IPI-O4 perturbs the self-association of IPI-O1 in planta. Total proteins were extracted from N. benthamiana plants expressing 4×Myc-IPI-O1 and 3×FLAG-IPI-O1 together with 3×HA-tagged IPI-O1 or IPI-O4. Immunoprecipitation was performed with an anti-Myc antibody, and immunoblots were probed with anti-FLAG, anti-Myc, or anti-HA antibodies.

(B) IPI-O4 enhances the association of RB CC with IPI-O1 in vivo. Total proteins were extracted from N. benthamiana plants expressing RB CC-4×Myc and 3×FLAG-IPI-O1 together with 3×HA-tagged IPI-O1 or IPI-O4. Immunoprecipitation was performed with an anti-Myc antibody, and immunoblots were probed with anti-FLAG, anti-Myc, or anti-HA antibodies. Ponceau S staining of immunoblots served as a loading control. The experiments were repeated three times with similar results.