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. Author manuscript; available in PMC: 2022 Nov 20.
Published in final edited form as: Neurosci Lett. 2021 Sep 30;765:136273. doi: 10.1016/j.neulet.2021.136273

Figure 3: Performance in novel object recognition and open field assays were not affected by PFF-induced synucleinopathy.

Figure 3:

In an open field assay, mice were allowed to explore in a 40 × 40 cm arena for 10 min, and ANY-maze software were used for tracking and video analysis. A) No significant differences in the percent of time spent close to the wall (an indicator of thigmotaxis, n=7, p=0.62, ranksum) or B) total distance travelled (n=7, p=0.80, ranksum), were noted between the PFF and control groups. C) Novel object recognition assays were performed 6 months following PFF injections into bilateral medial prefrontal cortex. Control mice received human synuclein monomers. Animals were familiarized with two identical objects for 10 min. One hour later, a novel object was introduced along with an identical copy of the familiar object, and a discrimination index was calculated as (NT−FT)/(NT+FT), where NT represents time spent with the novel object, and FT represents time spent with the familiar object. There was no significant difference between the PFF and control groups.