Table 1.
Mice produced through injection of round spermatids derived from transplanted spermatogonial stem cells into mature oocytes
| Round spermatid source | Recipient mice for SSC transplantation | Types of transplanted SSCs | Genetic background of SSCs | Number of injected oocytes | Number of transferred two-cell embryos | Number of live-born pup, n (%) | Number of pups with corrected Tex11 |
|---|---|---|---|---|---|---|---|
| Recipient mice | KitW/KitWV | HDR-1 | C57×DBA | 161 | 134 | 21 (15.67) | 20 |
| Recipient mice | KitW/KitWV | Tex11 PM/Y | C57×DBA | 155 | 36 | 0 (0) | 0 |
| Recipient mice | Busulfan-treated mT/mG | HDR-1 | C57×DBA | 140 | 120 | 22 (18.33) | 22 |
| Recipient mice | Busulfan-treated mT/mG | HDR-1 | C57×DBA | 145 | 124 | 18 (14.51) | 18 |
| Control | - | - | - | 150 | 130 | 24 (18.46) | 0 |
Tex11: testis-expressed 11; SSCs: spermatogonial stem cells; HDR: homology-directed repair; DBA: Dilute Brown Non-Agouti