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. 2021 Oct 25;78(6):1871–1884. doi: 10.1161/HYPERTENSIONAHA.120.17646

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© 2021 The Authors.

Hypertension is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Figure 2. — Biopterin levels, blood pressure, and fetal growth in pregnant mice with heterozygous deletion of Gch1 in endothelial cells (ie, Gch1^fl/+ Tie2cre mice). Mice with heterozygous deletion of Gch1 in endothelial cells (ie, Gch1^fl/+ Tie2cre mice) were generated by crossing Gch1^fl/fl Tie2cre mice with wild-type (WT; ie, Gch1^+/+) mice. Female Gch1^fl/+ Tie2cre mice were mated with WT male mice. A, Genomic polymerase chain reaction shows the presence of the Gch1 floxed and WT alleles in both Gch1^fl/+ (WT) and Gch1^fl/+ Tie2cre mice. B, Levels of tetrahydrobiopterin (BH4), and total biopterins were measured by high-performance liquid chromatography (HPLC) in tissue homogenates obtained from WT and Gch1^fl/+ Tie2cre mice, at the end of pregnancy. Total biopterins include BH4+dihydrobiopterin (BH2)+B. Shown are biopterin levels in aorta, pregnant uterine artery. *P<0.05 vs WT. C, Blood pressure and heart rate at baseline (prepregnancy; n=6–8 animals per group). D, Blood pressure and heart rate before and during pregnancy (n=6–8 animals per group). E, Litter size (number of embryos) was not different between WT and Gch1^fl/+ Tie2cre mice (n=6–8 animals per group). F, Fetuses at gestation day 18.5 were significantly smaller from pregnant Gch1^fl/+ Tie2cre compared with WT females. *P<0.05 vs WT; n=6–8 animals per group. B indicates biopterins.