Figure 3.

Validation of metabolites and NAFLD-HCC Diagnostic Score (NHDS). a. The relative abundances of 10 validated metabolites in discovery (blue) and validation (purple) sets are expressed as a fold change against CTRL. Metabolites included in NHDS are marked in red. b. ROC curves of NHDS in the discovery set. c. ROC curves of NHDS in the NAFLD-HCC validation set. d. The forest plot presenting odds ratios (Fisher's exact test) of NHDS at the established cut-off value as well as clinical and biochemical characteristics for discovery, validation, and pulled (discovery and validation) sets. AFP and GGT were available only in the discovery set. Error bars present 95% CI, bold font was used for statistically significant (Fisher's exact test, p<0•05) factors #The Odds ratio for NHDS in validation set was infinite e. The forest plot representing the relative risk (Fisher's exact test) of NHDS at the established cut-off value as well as clinical and biochemical characteristics for discovery, validation and pulled (discovery and validation) sets. AFP and GGT were available only in the discovery set. Error bars present 95% CI, bold font was used for statistically significant (Fisher's exact test, p<0•05) factors