FIG 7.
Tissue infiltration of NK cells and inflammatory monocytes is reduced during MAYV infection in mice previously infected with CHIKV. The C57BL/6 mice were mock or CHIKV infected, and after 28 days, mice were mock, CHIKV, or MAYV infected in the hind paw (n = 3 to 5 per group). After 7 days, mice were euthanized, and their feet removed and processed for cell immunophenotyping analysis. Representative contour plots showing the frequency of (A) NK cells (CD3-NK1.1+) and (B) inflammatory monocytes (CD11b+ Ly6Chigh) in the hind paws of each experimental group. The frequencies (B, D, G, and I) and absolute numbers (C, E, H, and J) of both cell subtypes are shown. Data are representative of two independent experiments. Statistical analysis was performed by one-way ANOVA with Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.