TABLE 1.
Summary of data collection and image-processing parameters
| Parameter | AAVrh.10-7x | AAVrh.10:LacNAc |
|---|---|---|
| Data collection statistics | ||
| Total no. of micrographs | 589 | 1,437 |
| Defocus range (μm) | 0.95–3.24 | 0.99–4.05 |
| Total electron dose (e−/Å2) | 75 | 60 |
| Frames/micrograph | 50 | 43 |
| Pixel size (Å/pixel) | 1.05 | 0.97 |
| No. of capsids used for final map | 17,861 | 142,036 |
| Resolution of final map (Å) | 2.71 | 3.09 |
| Refinement statistics | ||
| Map CC | 0.870 | 0.851 |
| RMSD | ||
| Bonds (Å) | 0.01 | 0.01 |
| Angles (°) | 0.82 | 0.86 |
| All-atom clash score | 7.52 | 8.20 |
| Ramachandran plot (%) | ||
| Outliers | 0 | 0 |
| Allowed | 1.4 | 1.5 |
| Favored | 98.6 | 98.5 |
| Rotamer outliers (%) | 0 | 0 |
| C-beta deviations | 0 | 0 |