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. 2021 Nov 9;95(23):e01323-21. doi: 10.1128/JVI.01323-21

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FIG 5 — Effect of the deletion of the cleavage site between PR and RT in GagPol on autoprocessing. (a to e) Diagrams indicate the structure of GagPol(WT) and variants. Numbers above the diagrams indicate cleavage sites 1 to 9 by mature PR. Molecular size of each domain: MA, 17 kDa; CA, 24 kDa; p2, 2 kDa; NC, 7 kDa; TF, 1 kDa; P (PR),10 kDa; RT, 51 kDa; RH, 10 kDa; IN, 35 kDa. Cleavage site 7 between PR and RT in HIV(WT) and HIV(IN:M50I) was deleted by point mutagenesis as described in Materials and Methods, and the resulting clones contain a fusion gene of PR and RT (PR-RT). (f to i) Virus particles are isolated using ultracentrifugation as described in Materials and Methods, and 5 μg of viral lysates was subjected to WB. PR, CA, and IN were detected by anti-PR (f), anti-MA (g), anti-CA (h), and anti-IN antibodies (i).