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Impact of mutations at codon 50 in IN on HIV replication. PHA-stimulated primary CD4+ T cells were infected with HIV(WT) or variants containing a different mutation at codon 50 as described in Materials and Methods. The infected cells were cultured for 14 days, changing medium every 3 to 4 days. HIV replication was monitored using a p24 antigen capture kit. Representative data from two independent assays are presented as means ± SD (n = 3).
