FIG 5.

Altered transcription of key peroxisome and interferon-related genes are observed following HPgV WT or ΔNS2 infection of human astrocytes and microglia. (A to J) Astrocytes were infected with HPgV WT or ΔNS2 (MOI, 0.1) or left uninfected, and cell lysates were harvested for RNA following 7 days of infection (n = 3 to 5). Select peroxisome- and interferon-related transcripts, including ABCD1 (A), ABCD3 (B), PEX3 (C), PEX5L (D), PEX7 (E), PEX11B (F), MAVS (G), IRF1 (H), IRF3 (I), and IFNB (J), were analyzed. Microglia were infected with HPgV WT or ΔNS2 (MOI, 0.1) or left uninfected, and cell lysates were harvested for RNA following 2 days of infection. Select peroxisome transcripts, including ABCD1 (K), ABCD3 (L), PEX3 (M), PEX5L (N), PEX7 (O), PEX11B (P), MAVS (Q), IRF1 (R), IRF3 (S), and IFNB (T), were analyzed. The time courses were assessed for IFNA (U) and IRF3 (V) transcript levels as well as LDH release (W) and cell viability (V) in astrocytes following infection by either WT and ΔNS2 viruses and compared to mock-infected cells (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ANOVA followed by Tukey’s post hoc test).