FIG 6.

HPgV positive- and negative-strand RNA and viral protein detection in human brain. (A) HPgV⁺ NS3 copies were detected in HPgV⁺ (n = 7) brain samples but not in HPgV⁻ (n = 6) cortex samples. (B) Similarly, HPgV⁻ E2 copies were detected in the same samples in HPgV⁺ (n = 7) and was not detected in HPgV⁻ (n = 6) samples. Frontal lobe tissue sections from HPgV⁺ or HPgV⁻ patients were immunolabeled for GFAP (red), Iba-1 (green), and viral protein HPgV E2 (purple) (C) or HPgV NS5A (D) and imaged using confocal microscopy.