TABLE 1.
Characteristics of the dengue patients from whom plasmablasts were sorted for RNA-seq analysis
| Patient | Age (yrs) | No. of days after onset of clinical symptoms | Disease severitya | Plasmablast frequency (%)b | No. of IgG ASCsc/106 PBMCs (% dengue specific) | No. of IgM ASCsc/106 PBMCs (% dengue specific) |
|---|---|---|---|---|---|---|
| 1 | 8.4 | 6 | SD | 58.7 | 0.55 × 106 (41.2) | 0.032 × 106 (2.5) |
| 2 | 11 | 6 | SD | 61.0 | 0.09 × 106 (70.4) | 0.002 × 106 (26.7) |
| 3 | 8.8 | 4 | SD | 49.3 | 0.066 × 106 (98) | 0.014 × 106 (2.8) |
| 4 | 11.6 | 4 | SD | 56.5 | 0.08 × 106 (60.1) | 0.012 × 106 (7.4) |
| 5 | 11.7 | 7 | DW | 33.4 | 0.076 × 106 (71.2) | 0.017 × 106 (5.6) |
| 6 | 11.2 | 7 | DW | 60.4 | 0.043 × 106 (58.3) | 0.0024 × 106 (0) |
| 7 | 9.5 | 7 | DW | 41.8 | 0.032 × 106 (48.1) | 0.0052 × 106 (6.9) |
| 8 | 12 | 5 | DW | 50.9 | 0.032 × 106 (66.7) | 0.012 × 106 (1.7) |
a
Disease grade classification was done according to 2009 WHO criteria (28). SD, severe dengue; DW, dengue with warning signs.
b
Percentage of B cells.
c
ASCs, antibody-secreting cells.