TABLE 3.
Bud morphology, septin organization, and pattern of chitin deposition in wild-type and mutant strainsa
| Strain | Bud morphologyb
|
Septin organizationc
|
Chitin depositiond
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Normal | Elongated | Wild-type | Fuzzy | Bars | Absent | Asymmetric | Symmetric | Delocalized | |
| Wild type | 97 | 3 | 95 | 5 | 0 | 0 | 97 | 3 | 0 |
| swe1Δ | 99 | 1 | 87 | 12 | 1 | 0 | 92 | 3 | 5 |
| mih1Δ | 96 | 4 | 90 | 9 | 0 | 1 | 96 | 4 | 0 |
| gin4Δ | 71 | 29 | 35 | 25 | 28 | 12 | 17 | 66 | 17 |
| gin4Δ swe1Δ | 97 | 3 | 23 | 35 | 32 | 3 | 11 | 76 | 13 |
| gin4Δ mih1Δ | 34 | 66 | 12 | 33 | 37 | 18 | 1 | 53 | 46 |
| cla4Δ | 31 | 69 | 31 | 32 | 19 | 18 | 9 | 50 | 41 |
| cla4Δ swe1Δ | 94 | 6 | 28 | 31 | 23 | 18 | 5 | 61 | 34 |
| nap1Δ | 84 | 16 | 41 | 36 | 13 | 10 | 9 | 78 | 13 |
| cla4Δ gin4Δ | 6 | 94 | 3 | 17 | 47 | 33 | 0 | 22 | 79 |
| cla4Δ gin4Δ swe1Δ | 96 | 4 | 8 | 33 | 33 | 26 | 0 | 28 | 72 |
| nap1Δ gin4Δ | 40 | 60 | 12 | 31 | 38 | 19 | 6 | 76 | 18 |
| nap1Δ gin4Δ swe1Δ | 98 | 2 | 27 | 35 | 27 | 12 | 8 | 82 | 10 |
| hsl1Δ | 94 | 6 | 89 | 11 | 0 | 0 | 95 | 5 | 0 |
| kcc4Δ | 96 | 4 | 95 | 5 | 0 | 0 | 96 | 4 | 0 |
| hsl1Δ kcc4Δ | 96 | 4 | 94 | 6 | 0 | 0 | 96 | 4 | 0 |
| gin4Δ hsl1Δ | 72 | 28 | 28 | 30 | 32 | 10 | 9 | 79 | 12 |
| gin4Δ hsl1Δ kcc4Δ | 73 | 27 | 22 | 35 | 35 | 8 | 10 | 82 | 8 |
| kcc4Δ mih1Δ | 97 | 3 | 90 | 7 | 2 | 1 | 92 | 8 | 0 |
| hsl7Δ | 94 | 6 | 92 | 8 | 0 | 0 | 95 | 5 | 0 |
All strains were grown to exponential phase in YM-P medium at 30°C, and ≥200 cells were counted per sample. The strains used were the wild type, YEF473; swe1Δ, M-1077; mih1Δ, M-600; gin4Δ, M-272; gin4Δ swe1Δ, M-825; gin4Δ mih1Δ, M-829; cla4Δ, M-515; cla4Δ swe1Δ, M-1029; nap1Δ, M-546; cla4Δ gin4Δ, M-522; cla4Δ gin4Δ swe1Δ, M-1025; nap1Δ gin4Δ, M-544; nap1Δ gin4Δ swe1Δ, M-976; hsl1Δ, M-601; kcc4Δ, M-286; hsl1Δ kcc4Δ, M-602; gin4Δ hsl1Δ, M-603; gin4Δ hsl1Δ kcc4Δ, M-604; kcc4Δ mih1Δ, M-1031; and hsl7Δ, M-1439.
Buds were scored as elongated if the length of the bud was more than twice the width of the mother cell.
Septin organization was scored as wild type (localization throughout the neck with well-defined end points; e.g., Fig. 3A), fuzzy (localization throughout the neck but with ill-defined end-points; e.g., Fig. 3D), bars (localization to several bars at the neck, oriented along the mother-bud axis; e.g., Fig. 3B and D), or absent (no localized staining seen), as described previously (40).
Chitin deposition was scored as asymmetric (chitin localized to the mother side of the neck, as in wild-type cells), symmetric (chitin localized with similar staining intensity on both mother and daughter sides of the neck), or delocalized (chitin dispersed more or less uniformly around the cell periphery), as described previously (40).