TABLE 5.

Effects of perturbing septin organization on the neck localization of Hsl1p and Hsl7p^a

Strain	% of cells with neck localization of:
	Septinsb	Hsl1p	Hsl7p
Wild type (M-1427)	96	92	NA
gin4Δ (M-1443)	94	25	NA
cla4Δ (M-1451)	91	27	NA
nap1Δ (M-1452)	90	23	NA
Wild type (M-1423)	100	NA	90
gin4Δ (M-1442)	86	NA	27
cla4Δ (M-1454)	86	NA	23
nap1Δ (M-1453)	89	NA	20

^aWild-type and homozygous mutant diploid strains were grown to exponential phase in YM-P medium at 23°C, and the localizations of septins, Hsl1p, and Hsl7p were determined by double-label immunofluorescence microscopy. More than 200 budded cells were counted per sample. NA, not applicable. 

^bNote that in the mutant strains, a majority of the cells with septins localized to the neck had abnormal septin organization, as illustrated in Fig. 3 and quantitated in Table 3 for strains closely related to those used for the experiments reported here.