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. 2021 Oct 26;8:728661. doi: 10.3389/fmolb.2021.728661

FIGURE 9.

FIGURE 9

BRD4-dependent genes with RSV-inducible chromatin. 7,845 RSV-inducible Tn5 transposase-accessible genomic cleavage sites were mapped to BRD4-dependent gene bodies. 516 peaks were mapped to BRD4-dependent genes. (A) Location of cleavage sites on gene bodies. (B) GO enrichment analysis. Bars, fraction of pathway in datasets. Scatterplots are false discovery rate for pathway enrichment. Note the enrichment of collagen trimers and fibril formation. (C) Integrated GO enrichment. A major network involved in ECM and collagen is indicated in green. (D) MMP9 mRNA expression in RNA-Seq is validated by qRT-PCR. Left, log2FC from RNA-Seq. Adjusted p-values is < E-100. Right, fold change mRNA by qRT-PCR. (E) mRNA expression of other ECM and structural index genes are plotted as 25–75% interquartile ranges. Adjusted p-values of RSV vs RSV + ZL0454 treatment are: COL17A = E < -100; MUC1 = 1.4E-76; SERPINA1 = E < -100; and CDH3 = 3.33E-236.