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. Author manuscript; available in PMC: 2021 Nov 9.
Published in final edited form as: J Proteome Res. 2020 Dec 28;20(2):1229–1242. doi: 10.1021/acs.jproteome.0c00604

Figure 4: TPR interactors are primarily nuclear localized.

Figure 4:

A, Subcellular fractionation of HeLa cells demonstrating localization of OGT (anti-OGT F12) and BirA* fusion proteins (anti-FLAG tag). Cytoplasmic marker is GAPDH, nuclear marker is Histone H3. 10ug/lane, representative western blot of three biological replicates B, Ratios of nuclear to cytoplasmic expression of marker proteins (Nuclear: Histone H3, Cytoplasmic: GAPDH) and fusion proteins. Averaged across three biological replicates. C, UpsetR plot showing the subcellular localization of TPR interactors. D, Venn diagram showing the subcellular localization of TPR interactors. Numbers represent the total number of TPR interactors in that category. Localization determined using UniProt.